![]() ![]() Many players arrived en masse to express their continued protest, support, and fond farewells, including messages of gratitude from the developers and moderators thanking their fans for their support and passion for the game. November 30, 2012, was listed as the official shutdown date of the game and the servers were turned off at midnight PST. The stated explanation for this move was a "realignment of company focus and publishing support". On August 31, 2012, Paragon Studios announced that it was being closed, and City of Heroes would cease all billing immediately and begin the process of shutting down the service. ![]() Special models for former subscribers would be termed Premium Players, and current subscribers would become VIP players, who would gain access to all the content in the various upcoming game updates. On June 20, 2011, Paragon Studios announced that they were going to switch to a hybrid subscription model called City of Heroes: Freedom, adding in a free-to-play game model. Going Rogue was released on August 17, 2010, with pre-purchasers able to play on August 16. Paragon Studios described this as " the shades of gray that lay between Heroes and Villains". ![]() This part of the game centered on the alternate reality of Praetoria and featured a new alignment system allowing players characters to shift allegiances between Heroes and Villains, giving characters access to both Paragon City of City of Heroes and the Rogue Isles of City of Villains. The City of Heroes: Going Rogue expansion's release was announced on May 11, 2009. On October 30, 2008, NCsoft announced a partnership with Transgaming Technologies in order to bring both City of Heroes and City of Villains and all 13 expansions to Mac OS X. This then led to City of Heroes becoming available for download on Steam, along with other NCsoft titles, on April 22, 2009. The new studio on April 14, 2009, became Paragon Studios, which shared credit with Cryptic Studios for the development work. On November 6, 2007, NCsoft announced their purchase of the City of Heroes/ City of Villains intellectual property and transitioned the staff from Cryptic Studios to a new location in Mountain View, California, to continue development of the game. Prior to this, a purchase was required to access either game's content, but they were linked by one account and subscription fee. Thus, a player who only owned City of Heroes could now play City of Villains, and vice versa. On July 16, 2008, NCsoft merged the two games' content together. The stand-alone expansion pack did not require City of Heroes to run, but if the user had both games, content was added to the City of Heroes side of game play. On October 31, 2005, the game's first sequel, City of Villains ( CoV), was launched, allowing players to play as supervillains. Even after resigning from Cryptic, Dakan remained involved with the game through the publishing of the City of Heroes comic book. Dakan was named Lead Design on the game, doing mostly writing for characters and story. The company's first project was City of Heroes, the idea for the game which Dakan claims to have come up with. In 2000, writer Rick Dakan and Michael Lewis created Cryptic Studios. As of October 2022, NCSoft has not moved to have servers based on this source code shut down. This made it possible to create City of Heroes servers outside the direct purview of NCSoft and revived interest in the game, which by then had been out of development for more than six years. In April 2019, source code capable of running a City of Heroes server was distributed widely. On August 31, 2012, NCsoft terminated its Paragon Studios development team, ending all production on City of Heroes with the last day of services on November 30, 2012. The final live update, "Where Shadows Lie", was released on May 31, 2012. Twenty-three free major updates for City of Heroes were released before its shutdown. In the game, players created super-powered player characters that could team up with others to complete missions and fight criminals belonging to various gangs and organizations in the fictional Paragon City. The game was launched in North America on April 28, 2004, and in Europe by NCsoft Europe on February 4, 2005, with English, German and French servers. City of Heroes ( CoH) was a massively multiplayer online role-playing game which was developed by Cryptic Studios and published by NCSOFT. ![]()
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![]() ![]() Using electron effective mass m and electron-transverse phonon coupling constant λ as two fitting parameters, we are able to describe quantitatively a large set of the measured temperature dependences of resistivity R(T) and Seebeck coefficient S(T) for a broad range of electron densities studied experimentally in recent paper by Collignon et al. We have used the kinetic equation approach within relaxation-time parametrization of the collision integral and we have determined energy-dependent transport relaxation time τ(E) by the iterative procedure. We developed a theory of electric and thermoelectric conductivity of lightly doped SrTiO3 in the nondegenerate region kBT≥EF, assuming that the major source of electron scattering is their interaction with soft transverse optical phonons present due to proximity to ferroelectric transition. ![]() The latter are reminiscent of Kondo effect and most probably due to oxygen vacancies. Sr $$(x)$$ n ≥ n * ( x ), resistivity follows a T-square behavior together with slight upturns (in both Ca-free and Ca-substituted samples). The fate of electric dipoles inside a Fermi sea is an old issue, yet poorly explored. Our observations agree qualitatively with a scenario where superconducting pairing is mediated by fluctuations of the ferroelectric soft mode. When approaching SrTiO318 the maximum Tc occurs at much smaller carrier densities than for pure SrTiO316. For carrier concentrations around 5×1019cm−3 this Tc increase amounts to almost a factor 3, with Tc as high as 580 mK for y=0.74. We show that the superconducting Tc increases when the O18 concentration is increased. Here, we explore the superconducting properties of doped and isotope-substituted SrTi(18Oy16O1−y)3−δ for 0≤y≤0.81 and carrier concentrations between 6×1017 and 2×1020cm−3 (δ<0.02). In undoped STO, ferroelectric order can in fact be stabilized by substituting O16 with its heavier isotope O18. The fact that superconductivity occurs at very low carrier concentrations is one of the two reasons that the pairing mechanism is not yet understood, the other being the role played by the proximity to a ferroelectric instability. Instead, it enhances the superconductivity directly, despite the absence of strong quantum fluctuations.ĭoped strontium titanate SrTiO3 (STO) is one of the most dilute superconductors known today. These results suggest that centrosymmetry breaking, i.e., the ferroelectric nature, does not kill the superconductivity. ![]() The enhancement then becomes much more prominent at locations further inside the dilute carrier-density region, where the screening is less effective. However, the T c enhancement is unexpectedly lower within the vicinity of the putative ferroelectric quantum critical point. The maximum T c reaches 0.75 K, which is the highest reported value among the SrTiO 3 -based families to date. Interestingly, the superconducting transition temperature T c is enhanced more strongly in these polar metals when compared with the nonpolar matrix Sr(Ti, Nb)O 3. Systematic substitution of Nb ⁵⁺ for Ti ⁴⁺ has revealed that these polar metals both commonly show a simple superconducting dome with a single convex shape. Keyboards are shown for demonstrative purposes only and are not included with the purchase of these keycaps.Two different ferroelectric materials, Sr 0.95 Ba 0.05 TiO 3 and Sr 0.985 Ca 0.015 TiO 3, can be turned into polar metals with broken centrosymmetry via electron doping. This item is in stock and will ship within a few business days from Germany. If for example you intend to mix the International kit from this round with a base kit from the previous round, be advised that there will be a small physical difference! See here and here.ĭesigned by biip with an all new font and icons. There was a mold change between the previous release (sold in 2021) and this one, the profile is now slightly lower. WARNING FOR MIXING NEW KITS WITH PREVIOUS RELEASE There is a :, key that can be used in its place. The International kit was mistakenly produced without a, key necessary for the German layout. ![]() ![]() ![]() With Lili Taylor, Pamela Z, Jim Lange, Evan White. The Weather Underground: Directed by Sam Green, Bill Siegel. Check out our finger-friendly friends and make sure to. เครื่องบินขับไล่ราฟาลชุดแรกถึงอินเดียแล้ว - การทูตและการทหาร Military&DiplomacyProfile of Bill Ayers, who has written memoir, some of it fiction, of his years as a member of Weather Underground, radical Vietnam-era group photo as youth, and with wife, Bernardine Dohrn (M)Weather Underground: Our Data 44K views8 years ago Finger-Friendly Weather Play all Playing with the weather has never been easier. Pivotal Weather is changing the way you use weather data. Want your own PWS? Check out our buying guide: .เครื่องบินขับไล่ราฟาลชุดแรกถึงอินเดียแล้ว – การทูตและการทหาร Military&Diplomacy Freely available fast quality numerical weather prediction model images from the Euro, GFS, HRRR, NAM, 3km NAM and more. I have a problem with Weather Underground Weather Underground outages reported in the last 24 hoursWeather Underground's Personal Weather Station Network takes on Gardening. Are you also having issues? Select the option you are having issues with, and help provide feedback to the service. Weather Underground is a commercial weather service providing real-time weather information via the Internet. St James's, England, United Kingdom 43 ☏. Schiller Park, IL (60176) 25 ☏ Light Snow. Weather Underground: Our Data 44K views8 years ago Finger-Friendly Weather Play all Playing with the weather has never been easier. Weather Underground is a commercial weather service providing real-time weather information over the Internet. Mike Bettes Chris Bruin Meteorologist Scot Pilie' 82 33 comments 19 sharesThe Weather Underground was a far-left militant organization described by the FBI as a domestic terrorist group 1970, their first publicly acknowledged bombing occurred at a New York City police station. We're detailing the multi-day severe risk and potential for blizzard conditions in the Plains today on Weather Underground. All hazards of severe weather on the table next week including damaging winds, large hail & tornadoes. ![]() Weather data & microclimate : Weather Underground. You’ll always be prepared for whatever the weather brings.Android Apps by Weather Underground on Google Play. Discover about current conditions, interactive Nexrad radar, get customizable severe weather alerts and much more. CL 08-3689 Site Manager: Stan SanderWeather Underground combines data from over 250,000+ personal weather stations and a proprietary forecast model to give you the most accurate and hyperlocal forecasts, at a microclimate level. + Privacy / Copyright JPL External Clearance No. Check out our weather underground selection for the very best in unique or custom, handmade pieces from our shops.Searchable Archived Data at Weather Underground. ![]() ![]() ![]() Houses were abandoned and fell into disrepair. Residents went into alleys to shoot up, and often never came out alive. iv Then, like adjacent Grays Ferry, Point Breeze was hit by the heroin epidemic of the 1970s and then the crack scourge of the early 1990s. Many Jewish shopkeepers sold their businesses and moved elsewhere, part of a pattern that repeated itself throughout the city of Philadelphia. The race riots of the 1960s - which triggered mass “white flight” –signaled the decline of Point Breeze as a self-sustaining, relatively integrated neighborhood. ![]() “If you had a problem,” Alice remembered, “you were told to ‘Go see Bill Barrett.’” During the 1950s, Wharton Square was the home of Congressman Bill Barrett, who made sure Point Breeze got its fair share of city services. The three story houses fronting the square were the largest in the neighborhood. Wharton Square, one of the few green spaces in the area, was a friendlier place for Point Breeze’s African-American community, popular with picnickers. They would often wait for a long time, then give up and go home. There was no “Whites Only” sign, but segregation at this movie theater was an unspoken rule.Īnd at the 26th and Morris playground, Alice and her friends would wait for the white kids to get off the swings. Alice stood in back, endured the tormenting, and never came back. But when she and her friends entered the theater, the white audience began harassing them. One day, she went to see a film at The Breeze, another theater on Point Breeze Avenue. Yet Alice realized she was not welcome in certain places. ![]() We were allowed to go as a group to the 1700 block of Point Breeze to buy water ice.” When she wanted to go to see a movie at the Victory or the Dixie on Point Breeze Avenue, her mother would give her 16 cents: 5 cents for a bag of pretzels, 10 cents for the movie, and a penny for the tax. “After church, we would look in the store windows and fantasize about what we could buy,” Alice remembered about her childhood. ![]() We knew one was white and one was black and that was it.”Ĭlaudia’s friend Alice Gabbadon, who grew up at 22nd and Dickinson, also had fond memories of life in Point Breeze. I could go to anyone’s house and eat a meal. “We had a beautiful community growing up. “It was a diversified community, with Caucasians and African-Americans living and working together,” she said. As a ten year old, Claudia took the lead in beautifying her block by planting the first flowerbox. The family had no refrigerator, indoor plumbing, or hot water until the early 1950s. “There used to be over a hundred stores on the Breeze.”Ĭlaudia spent her childhood in a rowhouse at 21st and Kater, just south of Fitler Square. “It was a very busy, beautiful area,” remembered Claudia Sherrod, whose parents came to Philadelphia from Georgia during the Great Depression. There were also kosher butcher shops that catered to the still-large Jewish community. There were two five and dime stores (Woolworth’s and Kresge’s), and the Curson family operated a dress shop patronized by residents for First Communion and weddings. At night, Point Breeze Avenue (known by residents as “The Breeze”) was illuminated by scores of shop signs advertising clothing, fresh produce, appliances, ice cream, and soda. Its residents almost never went into Center City, as they had everything they needed within a few blocks of their two story rowhouses. Until the late 1960s, Point Breeze was a relatively stable, self-sufficient neighborhood. DuBois as the “hereditary enemy” of urban African-Americans.” ii Many of Point Breeze’s African-Americans worked for Center City hotels, the Pennsylvania Railroad, local factories, and city government. This expansion often brought them into conflict with neighboring Irish-Americans, described by W.E.B. The Great Migration, however, pushed the boundaries of the African-American settlement west of Broad Street to Point Breeze. By the 1930s, these immigrant groups were joined by African-Americans from the Deep South, who had come to Philadelphia looking for work and to escape Jim Crow.ĭuring the nineteenth century, Philadelphia’s African-American community was centered east of Broad Street, near Mother Bethel African Methodist Episcopal Church at 8th and Lombard. i Conditions were primitive: chickens in backyards were a common sight. Italian and Irish immigrants soon followed. It was first settled by Eastern European Jews, many of whom set up shops on Point Breeze Avenue and lived in apartments above their businesses. Since the time of its beginnings in the late nineteenth century, Point Breeze has been a no-frills working class neighborhood. ![]() ![]() ![]() Macklemore's latest album, The Heist, is available on Amazon ( Vinyl, CD, MP3).įor Macklemore's latest music, news, and tour dates, check out his Zumic artist page. ![]() Growing Up (Sloanes Song) una canzone di. Scared of the process of staring at myself through a page and seeing someone that I. You can find a free download of the single on Macklemore's website, in addition to his open letter about growing up and being a dad: Leggi il Testo, scopri il Significato e guarda il Video musicale di Growing Up (Sloanes Song) di Macklemore. Sheeran holds down the chorus in his signature croon, with trumpets and choir-like accompanying vocals in the background. In a 2016 interview with Apple Music, Seattle rapper Macklemore recounted the moment when, at age 17, he realized his life’s true calling. With a simple drum beat, sporadic keyboard, and mild guitar strokes, Macklemore gives touching advice to his child while still addressing the fact that he feels he has a lot of growing up to do, too. He first started listening to rap at the age of 6 years old and has cited the famous rap collective, Digital. It's deeply personal and I'm proud of it."įrom the very first line of "They say boys don't cry / But your dad has shed a lot of tears," the song feels deeply intimate. Macklemore was born Benjamin Hammond Haggerty on June 19th 1983 in Seattle Washington. In an interview with MTV, Macklemore said, "It's one of my favorite songs I've ever written. The song reveals the hopes and fears of a soon-to-be father. Teaming up with producer Ryan Lewis and pop sensation Ed Sheeran, Macklemore has just released a new single, "Growing Up (Sloane's Song)." The track was written before the birth of his daughter, Sloane Ava Simone Haggerty. Macklemore dedicated Growing Up to his newborn daughter, Sloane, who was born on May 29, 2015. Thankfully, the latest news from Macklemore is definitely more on the cheerful side: He had a baby daughter this year. With a history of drug and alcohol abuse and a stint in rehab amidst a career that seemed to skyrocket over night, Macklemore has seen his fair share of ups and downs. On August 5, 2015, Macklemore released ‘Growing Up (Sloanes song)’, for free download, and after a few days, he launched a song ‘Downtown’. ![]() ![]() The scanning process can be paused or canceled at any time. This tool searches only the desired files by name, date, or size. It scans very fast the selected locations on the computer. This application tool has a modern interface that is easy to use by all users. You can also resort to it to clear duplicate playlist and library in Windows Media Player. It offers you alternative options to find duplicates by using the file size, file content, file name, last date modified, file extension, and file attributes. It features a fast search algorithm that finds duplicated file types including music, movies, pictures, and text. This interesting tool is used for fast search and deleting duplicates without the need of setting many options.ĪllDup is a tool for finding and removing duplicate files from a computer. It has multiple workspaces that can be saved as default when starting the program. ![]() This application has four modes for searching for files with the same content, same name, same name, and file and same content and name. This tool also has an internal preview that supports videos, audios, binary files, texts, and images.ĬloneSpy has a simple interface and compares two sets of multiple files by use of Pool 1 and Pool 2. Also, this tool allows you to search by similarity depending on the degree you choose. It has a preview area for viewing text files and playing audios before deleting them. Fast Duplicate File Finderįast Duplicate File Finder has one of the best user interfaces for duplicate finders and removers. The duplicate files found can either be deleted directly or moved to the recycle bin. This feature also allows you to search identical files with different extensions and the match percentage of files you want to be included. It a special file filter that excludes desired extensions and file sizes. Anti-TwinĪnti-Twin is a freeware which can search duplicate files and delete them. Top 5 tools to find and delete duplicate files on Windows 5. Luckily, there are effective tools that can be used to find and delete these duplicate files from your computer. Identifying duplicated files from the original ones and deleting them manually is almost close to impossible. Therefore, you need to keep a single file and clear the rest permanently. Whenever you modify one file in a certain location, all the duplicate files are modified at the same time. You don’t know which version to use since they are all duplicates. ![]() Having duplicate files on different locations of your computer is a total mess. To eliminate the need for duplicate file remover. ![]() To minimize time wasted while searching for files.To minimize the constant need for other storage media for backup.There are several benefits of deleting duplicated files from your computer. Duplicated files are mere junk and keeping them is just a waste of your hard disk space. You may not realize this until your computer starts running slowly. When you download any file from the internet, more often than not downloads come in with duplicated files. Proven solutions What are the benefits of deleting duplicate files?. ![]() ![]() New for 2021: cup and rod holders that can be mounted on the left or right side near the front console. Popular optional extras include a 15-litre fuel caddy ($200), a 20-litre removable storage box ($200), and an extra storage compartment that sits on top of the Fish Pro’s 51-litre cooler box ($349). The 2020 Sea-Doo Fish Pro 170 costs $20,499 not including registration and trailer, which typically add $1500 to $2000 depending on fees in each state and the type of trailer selected. This is a relatively small engine for such a large watercraft, but it was chosen to provide maximum riding range for a day of fishing activities. The larger capacity engine doesn’t need to work as hard to produce its higher output. In late 2019, Sea-Doo released the 2020 model which came with a non-supercharged 1630cc three-cylinder with a claimed output of 170 horsepower. The original Sea-Doo Fish Pro was powered by a non-supercharged 1503cc three-cylinder engine with an output of 155 horsepower. On other watercraft, riders need to lean over the handlebars to access the front storage area when on the water. The Sea-Doo Fish Pro is based on the company’s revolutionary ST3 hull, which has easy access to the front storage area because the handlebars lift up with the top of the centre console. Bluetooth audio speakers that are optional on other models are standard on Fish Pro models sold in Australia. ![]() It comes standard with a fish finder and navigation unit, a larger cooler box with rod holders, a long range fuel tank, and the capacity for a spare fuel caddy and other accessories. Unlike other personal watercraft, the Sea-Doo Fish Pro comes fully equipped ready to go, as a turn-key solution. It is based on the largest three-seater model in the Sea-Doo range. ![]() It was originally introduced in late 2018 as a 2019 model year but has been updated for 2020. Containers a bin organizer helps with the quick access items whilst a watertight compartment with integrated USB port allows your devices to stay charged-snug in an impact-absorbing, watertight cradle.The Sea-Doo Fish Pro is the world’s first dedicated fishing Jet Ski or personal watercraft. Simply raise the handlebars to access all the essentials for the day's adventures. DIRECT-ACCESS FRONT STORAGE: With watertight phone compartment - All your gear within arm's reach thanks to a 25.3 US gal (96 L) front storage area, easily accessible from a seated position.The ST3 platform offers easy boarding, plenty of space to move about on deck and superior confidence. STABLE AND PREDICTABLE HULL: Innovative Performance with room to Spare - The benchmark for rough water handling, stability, and offshore performance.Plenty of top-end speed to get on the fish fast and highly efficient, all-day fun and excitement are always on tap. The Rotax® 1630 ACE™ - 170 is the most powerful naturally aspirated Rotax engine ever on a Sea-Doo watercraft. ![]()
![]() ![]() PMI® is a registered mark of the Project Management Institute, Inc. PMBOK® is a registered mark of the Project Management Institute, Inc. ![]() Learn more about PMI’s industry leading certifications and see which one is right for you. Wherever you are in your career, PMI has a certification for you. Project Management Professional (PMP)® is a registered mark of the Project Management Institute, Inc. The Simplest Guide to Project Management and PMI-PMP Certification Exam Video Instant access to this title and 7,500+ eBooks & Videos Constantly updated. With a PMI certification behind your name, you can work in virtually any industry, anywhere in the world, and with any project management methodology. PMI is making changes to its PMP exam and content delivery. (Project Management Institute, Inc., 2017) and other appropriate PMI® global standards. The Project Management Professional (PMP) certification is recognized worldwide as one of the most valuable certifications for professionals in IT and many. The content and subject matter presented in this class are aligned with A Guide to the Project Management Body of Knowledge (PMBOK® Guide)-Sixth Edition PMIs Project Management Professional (PMP) credential is the most important industry-recognized certification for project managers. ![]() The course applies what you learned in the Project. Prepare to take the PMP® exam from the Project Management Institute (PMI)® to attain PMP® certification. The PMP Exam Preparation course is two half-days focused specifically on passing the PMP or CAPM exams. The PMP exam is based upon the Exam Content Outline Document and incorporates questions on predictive, agile, and hybrid approaches. Global standard, including the project management knowledge areas, processes, and best practices for the initiation, planning, execution, control, and closure of a project initiative. This advanced project management course is designed to help project managers master the current PMBOK® Guide Home PMP Student 20230327 27-31 March 2023 Port Hueneme Vets2PM CAPM® PMP® Exam Prep Boot Camp Plus Program 20230327 27-31 March 2023 Port Hueneme Vets2PM CAPM® PMP® Exam Prep Boot Camp Plus Program. Not only are project managers with Project Management Professional (PMP)® credentials more marketable, but they also have access to better opportunities. Vets2PM CAPM® PMP® Exam Prep Boot Camp Plus Program. In today’s competitive job market, credentials matter more than ever. PMP PMBOK: Audio Study Guide PMP Exam Prep Professional Exam Study Guide: Audio Crash Course & Master Test Prep to Help You Pass the Exam Practice Questions. ![]() ![]() ![]() This application lets you pay for utility bills using your Android phone or tablet. including the Highway 11 corridor and the land between Covington and Newborn. Mobile application for Newton County Water and Sewerage Authority, powered by Paymentus. The position is full time, salaried and falls under the Exempt status as defined by the Fair Labor Standards Act. County Board of Education and the Newton County Water and Sewerage. ![]() We are professional, innovative and our goal is to Provide our customers with clean safe drinking water while reclaiming wastewater in an environmentally sound manner while focusing on cost efficiency & value oriented services. The rent is including utilities (water, sewer and garbage) The unit has 2 bath. Newton County Water & Sewerage Authority is a small business in Covington, GA. Room near: Covington GA, Covington Newton County GA, Porterdale GA. ![]() ![]() ![]() The pipelines and images for these examples, as well as others, are available for download ( The image of yeast colonies ( Figure 1) is a plate of Hi90-strain cells plated on synthetic defined medium with 128 µg/mL fluconazole as previously described ( 5). Here we describe its use for a variety of other applications such as yeast colony counting, grid analysis, wound healing, and other visually quantifiable assays.Īll of the image analysis in this paper used the freely available CellProfiler cell image analysis software. ![]() We recently described CellProfiler's use for cell identification, cell size, intensity and texture of fluorescent stains, cell cycle distributions, and other features of individual cells in images ( 4). There are many existing software packages available for specific applications in biology, but CellProfiler accomplishes many of the same goals in one open-source program. The compiled software is freely available for Macintosh®, PC, and Unix platforms at It can accommodate adaptation to many biological objects and assays without requiring programming, due to its modular design and graphical user interface. ![]() The CellProfiler™ project was developed to address these software challenges by providing the scientific community with an easy-to-use open-source platform for automated image analysis. While users can program custom algorithms or record macros, these customized routines are challenging to adapt without knowing a programming language or interacting directly with the macro code. At the other end of the continuum, some software packages are very flexible, especially for interactive analysis of individual images. Most commercial software is proprietary, meaning that the underlying methods of analysis are hidden from the researcher. Other packages are sold with accompanying hardware for image acquisition (e.g., yeast colony counters), but these are expensive and do not allow measurement of features beyond those that are already built-in. While numerous commercial and free software packages exist for image analysis, many of these packages are designed for a very specific purpose, such as cell counting ( 2). Efforts to automate visual analysis in biology began several decades ago, but many aspects still need improvement ( 1). ![]() This liberates biologists for more interesting work and has several advantages over visual observations including speed, quantitative and reproducible results, and simultaneous measurement of many features in the image. Certain repetitive tasks in visual analysis are suitable for automation by collecting digital images and processing them with image analysis software. While nothing can fully replace the expertise of a trained biologist, observing many samples by eye is time-consuming, subjective, and nonquantitative. One of the most powerful methods in biology is the visual analysis of a sample. This free, easy-to-use software enables biologists to comprehensively and quantitatively address many questions that previously would have required custom programming, thereby facilitating discovery in a variety of biological fields of study. Small numbers of images can be processed automatically on a personal computer, and hundreds of thousands can be analyzed using a computing cluster. The software automatically identifies objects in digital images, counts them, and records a full spectrum of measurements for each object, including location within the image, size, shape, color intensity, degree of correlation between colors, texture (smoothness), and number of neighbors. The applications demonstrated here include yeast colony counting and classifying, cell microarray annotation, yeast patch assays, mouse tumor quantification, wound healing assays, and tissue topology measurement. Here we describe the use of the open-source software, CellProfiler™, to automatically identify and measure a variety of biological objects in images. Careful visual examination of biological samples is quite powerful, but many visual analysis tasks done in the laboratory are repetitive, tedious, and subjective. ![]() |